咨询电话

18612924577,18511767098

当前位置:首页   >  产品中心  >  ATCC  >  ATCC细胞  >  ATCCPRA-302WA1 ATCC细胞代理

WA1 ATCC细胞代理

简要描述:北纳生物经营范围:国家标准物质、国内外标准品、对照品、实验耗材、试剂、菌株、细胞、ATCC、培养基、血清、冶金标样、标准气体、试剂盒等,欢迎查询!ATCCPRA-302 WA1 ATCC细胞代理

  • 产品型号:ATCCPRA-302
  • 厂商性质:生产厂家
  • 更新时间:2019-04-17
  • 访  问  量:1462

相关文章

Related Articles

详细介绍

 公司介绍

  北京北纳创联生物技术研究院是一家专业从事标准物质、计量分析仪器和精细化工产品研发及销售的科研单位。是国内标准物质提供商之一,代理销售国内外数万种标准物质、微生物、菌种、细胞类等产品。

  北京标准物质网隶属于北京北纳创联生物技术研究院,具备强大的分析测试、技术开发、*、物流管理和售后服务能力,并与国内外众多检测机构、科研院校和工厂企业有着良好和稳定的合作关系,具有较强的性和影响力。

  北纳生物也隶属于北京北纳创联生物技术研究院,实验基地总占地面积16亩,并设有细胞库、微生物菌种保藏库,细胞培养、菌种培养等实验室。拥有细胞和菌株2万余株,并设有生物资源信息库,收录信息达20余万株。致力于提供实验室综合解决方案,为广大客户服务。

经营范围:

  国家标准物质、国内外标准品、对照品、实验耗材、试剂、菌株、细胞、ATCC、培养基、血清、冶金标样、标准气体、试剂盒等,欢迎查询!

ATCCPRA-302 WA1 ATCC细胞代理

基本信息
资源编号ATCCPRA-302
资源名称WA1
种属Babesia duncani
分离基物Human blood, Washington State, 1991
提供形式frozen
安全等级2
模式菌株no
应用领域Vector borne research
培养方法
生长条件Temperature: 25°C
Culture System: in vivo c*tion, hamster
存储条件Frozen Cultures:-70°C for 1 week; liquid N2 vapor for long term storageFreeze-dried Cultures:2-8°CLive Cultures:See Protocols section for handling information;Reagents Alsever's Solution NaCl, 4.2 g Na3citrate·2H2O, 8.0 g Glucose, 20.5 g Glass distilled H2O to 1.0 L *Dissolve components in glass distilled H2O, adjust the pH to 6.1 with 10% (w/v) citric acid and filter sterilize. The solution can be obtained from Sigma-Aldrich (cat# A3551). Harvest and Preservation Prepare a 30% (v/v) sterile glycerol solution in Alsever's solution. Draw approximately 0.5 mL of anticoagulant solution (Yaeger's or heparin, etc.) into a syringe and move it back and forth over the length of the syringe, several times. Remove all air bubbles. Draw blood by cardiac puncture into the syringe from a host animal that has reached or is near peak parasitemia. If clotting occurs during extraction of blood, insufficient heparin was used. Mix the heparinized blood with the 30% glycerol solution in a 2:1 ratio. If any clotting has occurred do not use. After mixing, the final concentration of cryoprotectant solution will be 10% (v/v). The mixture should be placed in a 4°C ice bath. The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Filled ampules should be placed in a 4°C ice bath. Do not immerse ampules to the level of the vial cap. Plunge ampules from 4°C into liquid nitrogen. The frozen preparations may be stored in a mechanical freezer until needed, however storage in either the vapor or liquid phase of a nitrogen refrigerator is recommended for the longest viability. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2 to 3 min). Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule. Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected hamster. Follow the protocol for maintenance in vivo. The course of infection may be longer or shorter than usual depending on percent recovery of the parasite from the frozen state.
详细说明
描述Strain has been passaged in vivo in jirds several times
Year of Origin1991

ATCCPRA-302 WA1 ATCC细胞代理

温馨提示:

  本店商品种类众多,因铺位有限商品未全部上架,如果没有找到您想要的商品可以咨询客服,我们会耐心解答,祝您购物愉快!

产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
扫一扫,关注微信
©2024 北京北纳创联生物技术研究院版权所有 All Rights Reserved.     备案号:京ICP备12027104号-6

技术支持:化工仪器网     管理登陆     sitemap.xml